FastQCFastQC Report
Thu 2 Jun 2016
DRR015291_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015291_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9908974
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA292400.038.3810041
GTATCAA473950.035.1733861
ATCAACG600200.027.5938573
TCAACGC601950.027.5576464
TATCAAC613750.027.0248132
CAACGCA642450.025.7664745
AACGCAG658750.025.2428596
GTACATG487600.024.3933411
TACATGG471100.023.9252952
ACGCAGA714500.023.1173237
CGCAGAG726900.022.498928
ACATGGG506550.021.7823753
GCAGAGT750950.021.2794029
GAGTACT379000.020.2855612-13
AGAGTAC628550.019.7897810-11
AGTACTT382150.019.43696212-13
GTACTTT391350.019.3683714-15
CATGGGG310750.019.110524
TACCTGG72150.018.8614162
CAGAGTA731900.017.84552610-11
Produced by FastQC (version 0.11.5)