FastQCFastQC Report
Thu 2 Jun 2016
DRR015291_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015291_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9908974
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371460.3748723127137078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302820.3056017706777715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173820.17541674849484923No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA348600.038.507371
GTATCAA548050.034.5237921
ATCAACG679650.027.6755623
TCAACGC682550.027.5179864
TATCAAC694450.027.1526172
CAACGCA703500.026.6791115
AACGCAG721450.026.0720716
GTACATG514950.024.6162471
TACATGG500850.024.1875362
ACGCAGA798300.023.3741467
CGCAGAG812750.022.801868
GCAGAGT835450.021.7738389
TACCTGG74250.021.7500382
ACATGGG541250.021.658223
GAGTACT427450.021.6145212-13
GTACTTT441750.020.7204314-15
AGAGTAC690400.020.25303810-11
AGTACTT430650.020.13846212-13
CATGGGG339050.019.534574
CAGAGTA815600.018.5357810-11
Produced by FastQC (version 0.11.5)