FastQCFastQC Report
Thu 2 Jun 2016
DRR015290_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015290_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6704076
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA206500.055.383551
GTATCAA403300.041.06351
ATCAACG523000.031.5918733
TCAACGC531950.031.1437914
TATCAAC541000.030.5857982
CAACGCA570050.029.0710855
AACGCAG583100.028.4810336
ACGCAGA631300.026.185377
CGCAGAG632300.026.0396868
GTGGTAT77950.024.9347651
GCAGAGT663800.024.4853389
TGGTATC78500.023.91492
TACCTGG42700.023.4826582
GTACATG471650.023.1993031
TACATGG449350.023.0519332
TCTGTCG20450.022.652358
GAGTACT391700.021.6611712-13
ACATGGG472150.021.48463
CATGGGT72650.021.023464
AGTACTT392450.020.917112-13
Produced by FastQC (version 0.11.5)