FastQCFastQC Report
Thu 2 Jun 2016
DRR015290_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015290_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6704076
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT369770.5515599763487168No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306870.45773645764159No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181430.2706264069798731No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT75740.11297604621427323No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70590.10529415239326045No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA210800.055.0058631
GTATCAA409150.041.963871
TATAACG4050.032.5714072
ATCAACG523750.032.4811483
TCAACGC534050.031.8376644
TATCAAC545850.031.3417722
CAACGCA558600.030.4628525
AACGCAG576400.029.6799496
ACGCAGA639050.026.6278847
CGCAGAG639100.026.5477098
GTGGTAT83450.025.452011
GCAGAGT676550.024.8899239
TGGTATC84650.024.7723662
TACCTGG44400.024.075672
GTACATG481250.023.8003981
TACATGG455250.023.6705652
TCTGTCG19500.023.3266548
ACATGGG477850.022.0365313
GAGTACT392250.021.8555712-13
AGTACTT393450.021.39569512-13
Produced by FastQC (version 0.11.5)