FastQCFastQC Report
Thu 2 Jun 2016
DRR015289_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015289_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8390706
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA203050.054.7277951
GTATCAA386450.042.2010881
ATCAACG516900.031.4218583
TCAACGC521300.031.1651124
TATCAAC524500.030.9931012
CAACGCA564500.028.7501955
AACGCAG578550.028.1283326
GTGGTAT76550.027.2439291
ACGCAGA624250.025.9012327
TGGTATC77700.025.7038482
CGCAGAG631050.025.4348378
GTACATG467800.024.142021
GCAGAGT658250.023.9483269
TACATGG449150.023.8758642
ACATGGG483250.021.6481043
GAGTACT373150.020.56772612-13
CAGAGTA637700.020.11494410-11
AGTACTT372200.020.08663212-13
AGAGTAC616050.020.07680510-11
GTACTTT385650.019.57993714-15
Produced by FastQC (version 0.11.5)