FastQCFastQC Report
Thu 2 Jun 2016
DRR015289_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015289_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8390706
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370310.44133354213578685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302640.36068478623848815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174850.2083853253826317No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA225650.057.9707451
GTATCAA425200.043.0596851
ATCAACG556150.032.6429523
TCAACGC561550.032.345254
TATCAAC569550.032.0122532
CAACGCA590000.030.762425
AACGCAG605350.030.0500016
GTGGTAT77800.028.5892331
ACGCAGA671100.026.8279917
TGGTATC82250.026.6582412
CGCAGAG680100.026.3326688
GCAGAGT711800.024.7381639
GTACATG481450.024.074511
TACATGG460150.023.8747082
GAGTACT418500.021.76242612-13
ACATGGG494250.021.5085753
TACCTGG61850.021.47652
CAGAGTA691450.021.2095410-11
AGTACTT420300.020.78686512-13
GTACTTT435050.020.7211814-15
Produced by FastQC (version 0.11.5)