FastQCFastQC Report
Thu 2 Jun 2016
DRR015288_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015288_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9610769
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA406800.029.4418331
GTATCAA602900.028.7841721
GTACATG462050.023.867091
TCAACGC729150.023.6794554
ATCAACG729900.023.6611673
TACATGG453500.023.400792
TATCAAC741900.023.276992
CAACGCA764350.022.5725865
AACGCAG778250.022.2544756
ACATGGG478050.021.413783
ACGCAGA838800.020.5361777
GAGTACT402050.020.5194812-13
CGCAGAG843850.020.356328
AGAGTAC635750.020.06611310-11
TACCTGG68650.019.9983062
AGTACTT401250.019.75798212-13
GTACTTT415350.019.6812914-15
GCAGAGT865400.019.3552889
CATGGGG286300.019.0183354
CATGGGT84150.017.5134244
Produced by FastQC (version 0.11.5)