FastQCFastQC Report
Thu 2 Jun 2016
DRR015288_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015288_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9610769
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT381790.3972522906335591No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313170.3258532173648123No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT207830.21624700375172892No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188850.1964983239114373No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT97410.10135505285789306No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA687800.028.569681
GGTATCA473800.027.841521
GTACATG480200.023.9963051
ATCAACG818900.023.7973293
TCAACGC820250.023.7304344
TACATGG467400.023.7287582
TATCAAC838450.023.357482
CAACGCA843200.023.0953335
AACGCAG857650.022.7327376
ACATGGG502300.021.6262283
GAGTACT451050.021.57356312-13
TACCTGG70150.021.5298182
ACGCAGA937350.020.6688127
GTACTTT466300.020.64020214-15
CGCAGAG945400.020.4303728
AGAGTAC696650.020.36517710-11
AGTACTT455650.020.32218412-13
GCAGAGT973700.019.5095489
CATGGGG303000.019.4718384
CATGGGT87750.018.4549354
Produced by FastQC (version 0.11.5)