FastQCFastQC Report
Thu 2 Jun 2016
DRR015287_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015287_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11679454
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA369400.035.6761781
GTATCAA597750.033.4735071
ATCAACG739000.026.936363
TCAACGC739650.026.9187684
TATCAAC758350.026.2730052
CAACGCA792950.025.1055165
AACGCAG808700.024.6493056
GTACATG550150.023.457331
ACGCAGA863350.022.8610367
TACATGG534700.022.6397382
CGCAGAG876300.022.3530658
GCAGAGT901250.021.240439
ACATGGG567200.020.8921953
GAGTACT441350.020.52330412-13
AGTACTT439550.020.09567612-13
GTACTTT452000.019.83900514-15
AGAGTAC722900.019.79837810-11
CATGGGG356600.018.025794
CAGAGTA879450.017.45246310-11
GTATTAG65050.017.307081
Produced by FastQC (version 0.11.5)