FastQCFastQC Report
Thu 2 Jun 2016
DRR015287_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015287_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11679454
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT381190.3263765583562382No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313820.26869406737677975No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180090.15419385187013024No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT146040.1250400917714133No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA427900.033.99911
GTATCAA651550.031.9749811
ATCAACG802200.025.676343
TCAACGC804400.025.6061174
TATCAAC825700.025.0731432
CAACGCA837800.024.5852955
AACGCAG855650.024.1149436
GTACATG584150.023.7345831
TACATGG562050.023.002412
ACGCAGA934700.021.9392137
CGCAGAG952850.021.3589948
GAGTACT463850.021.1687312-13
ACATGGG597050.021.0437133
GCAGAGT982600.020.3882489
GTACTTT477500.020.2743914-15
AGAGTAC763400.020.25609610-11
AGTACTT464250.020.21939812-13
CATGGGG379800.018.8880044
TACCTGG81700.018.5972462
GAGTACA441750.017.467361
Produced by FastQC (version 0.11.5)