FastQCFastQC Report
Thu 2 Jun 2016
DRR015286_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015286_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9364516
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA332550.034.365551
GTATCAA534450.033.210481
ATCAACG660250.026.7213843
TATCAAC664100.026.5877552
TCAACGC669550.026.3503634
GTACATG459450.023.653641
TACATGG454150.023.2108022
CGCAGAG762050.023.0618238
ACGCAGA765100.023.0558577
GAGTACT399950.022.42877812-13
GCCCATA123300.021.9455031
ACATGGG466200.021.8224223
CAACGCA812900.021.7043345
GCAGAGT799900.021.6287469
AACGCAG821750.021.5350116
AATGCGG46050.021.451517
AGTACTT414700.021.20032712-13
TACACGC79600.020.729595
TTACACG80100.020.544454
CATGGGT66450.020.249914
Produced by FastQC (version 0.11.5)