FastQCFastQC Report
Thu 2 Jun 2016
DRR015285_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015285_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8528980
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA202500.054.236181
GTATCAA389500.042.8634381
TCAACGC509650.032.67754
ATCAACG508800.032.653933
TATCAAC514600.032.374732
CAACGCA542100.030.690275
AACGCAG558050.029.9793786
GTGGTAT75300.027.0687661
ACGCAGA617600.026.8547747
CGCAGAG630350.026.2080178
TGGTATC77700.025.4361612
GCAGAGT652500.024.6618449
GTACATG459450.023.144051
TACATGG444300.023.0377332
TACCTGG62900.022.2832
GAGTACT369000.020.9189712-13
ACATGGG480150.020.7338053
CAGAGTA630550.020.53262310-11
AGTACTT373750.020.07399412-13
AGAGTAC610400.019.998310-11
Produced by FastQC (version 0.11.5)