FastQCFastQC Report
Thu 2 Jun 2016
DRR015285_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015285_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8528980
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390180.4574755715220343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314940.36925869212965673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194860.22846811693778155No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA218650.055.2180181
GTATCAA415950.044.629461
ATCAACG548100.033.6292343
TCAACGC548400.033.5527734
TATCAAC555650.033.2142
CAACGCA567950.032.373795
AACGCAG585950.031.50356
GTGGTAT80450.029.2329041
ACGCAGA657950.027.7794957
CGCAGAG672050.027.0477548
TGGTATC86200.026.439182
GCAGAGT701800.025.5516099
TACCTGG65400.023.3710942
GTACATG473400.023.0485821
TACATGG460500.022.7401432
GAGTACT418950.021.56483712-13
CAGAGTA680650.021.0783910-11
AGAGTAC661550.020.8926510-11
AGTACTT420000.020.85546912-13
GTACTTT433000.020.57713714-15
Produced by FastQC (version 0.11.5)