FastQCFastQC Report
Thu 2 Jun 2016
DRR015284_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015284_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9262679
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA213800.052.445521
GTATCAA413300.042.298511
ATCAACG534500.032.5416533
TCAACGC537150.032.3975524
TATCAAC544400.032.000832
CAACGCA577000.030.1230815
AACGCAG594200.029.273426
ACGCAGA645450.026.753967
CGCAGAG648650.026.473448
GCAGAGT677500.024.8969219
GTACATG454650.023.5451071
TACATGG439800.023.0256562
GTGGTAT88850.022.8233681
TGGTATC86750.022.511552
ACATGGG461600.021.3324363
GAGTACT397650.020.73783512-13
CAGAGTA662650.020.27014710-11
AGAGTAC634250.020.23145110-11
GTACTTT405050.019.88440714-15
AGTACTT398800.019.84803812-13
Produced by FastQC (version 0.11.5)