FastQCFastQC Report
Thu 2 Jun 2016
DRR015284_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015284_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9262679
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT374380.4041811229774885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306560.3309625649339678No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178060.19223380190547465No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA237750.055.713321
GTATCAA452000.042.8481641
ATCAACG573350.033.488973
TCAACGC577700.033.2368054
TATCAAC592150.032.5877652
CAACGCA604000.031.7443855
AACGCAG622550.030.8934946
ACGCAGA689550.027.5882767
CGCAGAG695750.027.1724368
GCAGAGT729550.025.5334689
GTGGTAT89450.025.4275281
GTACATG475200.025.0350271
TACATGG455150.024.6064622
TGGTATC92000.023.5361162
ACATGGG476650.022.742113
GAGTACT442400.022.32470712-13
CAGAGTA715100.021.36398710-11
GTACTTT460300.021.09175514-15
AGAGTAC682950.020.65766510-11
AGTACTT446800.020.53139512-13
Produced by FastQC (version 0.11.5)