FastQCFastQC Report
Thu 2 Jun 2016
DRR015283_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015283_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9788268
Sequences flagged as poor quality0
Sequence length97
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA217850.054.067681
GTATCAA422950.042.8908921
TCAACGC565900.032.0109984
ATCAACG563250.031.981323
TATCAAC576500.031.3047432
CAACGCA615600.029.4148275
AACGCAG631150.028.7880636
GTGGTAT81600.027.071671
ACGCAGA678050.026.6134877
CGCAGAG692050.025.9732078
GCAGAGT719050.024.4399159
TGGTATC86100.024.4242462
GTACATG528850.024.2437671
TACATGG514300.023.7174112
ACATGGG557050.021.352113
GAGTACT413150.020.88349512-13
AGAGTAC677850.020.42409710-11
AGTACTT413650.020.35146512-13
CAGAGTA696250.020.35023310-11
GTACTTT425900.019.95216814-15
Produced by FastQC (version 0.11.5)