FastQCFastQC Report
Thu 2 Jun 2016
DRR015283_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015283_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9788268
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT457370.4672634627494875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT367470.375418817711162No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225010.2298772367082716No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA247900.057.2685741
GTATCAA466700.043.241891
ATCAACG614900.032.5647433
TCAACGC621350.032.2559854
TATCAAC628100.031.9825762
CAACGCA651200.030.7704375
AACGCAG667150.030.0552436
ACGCAGA742550.026.7460827
CGCAGAG759500.025.9515328
GTGGTAT89350.025.708151
GCAGAGT792600.024.4031419
GTACATG563800.023.5154631
TGGTATC98850.023.1475242
TACATGG548500.023.0973282
TACCTGG71550.021.7435112
GAGTACT453300.021.71699712-13
AGTACTT452300.020.99541312-13
CAGAGTA767900.020.97849810-11
GTACTTT465650.020.93720214-15
ACATGGG593450.020.7187733
Produced by FastQC (version 0.11.5)