FastQCFastQC Report
Thu 2 Jun 2016
DRR015282_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015282_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9326555
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA232700.054.3080831
GTATCAA450950.042.6680221
TATCAAC593750.032.2438552
ATCAACG597200.032.069083
TCAACGC598100.032.050184
CAACGCA643850.029.7468035
AACGCAG661100.029.1108676
ACGCAGA726750.026.2780367
GTGGTAT89900.026.0410921
CGCAGAG736300.025.8298848
TGGTATC89550.025.5520572
GCAGAGT762650.024.4284159
GTACATG543800.023.9134161
TACATGG535750.023.4162772
ACATGGG577150.021.2500693
GAGTACT432600.020.81939712-13
AGTACTT431350.020.47563712-13
CAGAGTA739600.020.23195810-11
AGAGTAC714450.020.16284610-11
GTACTTT448100.019.96581614-15
Produced by FastQC (version 0.11.5)