FastQCFastQC Report
Thu 2 Jun 2016
DRR015282_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015282_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9326555
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT432850.46410491333616755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348360.37351412177379534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212560.22790837560063712No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA249000.055.755261
GTATCAA467900.042.9674221
TATCAAC615350.032.6248862
ATCAACG617450.032.482713
TCAACGC620800.032.226824
CAACGCA645450.031.0383555
AACGCAG666650.030.085436
ACGCAGA746000.026.7084857
GTGGTAT97100.026.4267921
CGCAGAG761100.026.0352928
TGGTATC98300.024.8980882
GCAGAGT795900.024.4913839
GTACATG554600.024.2271821
TACATGG547000.023.6692682
ACATGGG583150.021.6313483
GAGTACT451850.021.53097212-13
AGTACTT451600.021.07938812-13
TACCTGG74400.021.0340422
CAGAGTA772300.020.6215310-11
AGAGTAC742700.020.61334810-11
Produced by FastQC (version 0.11.5)