FastQCFastQC Report
Thu 2 Jun 2016
DRR015281_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015281_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8254419
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA189350.053.9975281
GTATCAA365800.042.3307881
ATCAACG478150.032.1499063
TCAACGC482000.031.8931054
TATCAAC487250.031.6073632
CAACGCA519150.029.6376195
AACGCAG532000.028.9632326
ACGCAGA580550.026.2370837
GTGGTAT75150.026.0940911
CGCAGAG586400.025.84738
GTACATG465500.024.5451051
GCAGAGT608800.024.3537379
TACATGG449900.024.2868882
TGGTATC77600.023.847372
ACATGGG478700.022.3029773
GAGTACT323350.020.67644912-13
TACCTGG57950.020.2729232
CAGAGTA592800.020.21553210-11
AGAGTAC571250.020.15956310-11
AGTACTT324000.020.06990412-13
Produced by FastQC (version 0.11.5)