FastQCFastQC Report
Thu 2 Jun 2016
DRR015281_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015281_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8254419
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264790.3207857512442729No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213380.25850396012123933No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126430.1531664433317475No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA199050.052.6189231
GTATCAA368200.041.616741
ATCAACG479700.031.7460943
TCAACGC484250.031.4665934
TATCAAC494800.030.8787822
CAACGCA507600.030.0191085
AACGCAG522650.029.215616
ACGCAGA583650.025.9595227
CGCAGAG593050.025.4408368
GTACATG480500.024.7287271
TACATGG461800.024.5948662
GCAGAGT623350.023.576949
GTGGTAT85500.023.135441
ACATGGG496000.022.284313
TGGTATC86000.022.0555272
GAGTACT323750.021.12503412-13
AGTACTT326300.020.81351712-13
AGAGTAC576450.020.39722310-11
GTACTTT330800.020.31177114-15
CAGAGTA602300.019.85414710-11
Produced by FastQC (version 0.11.5)