FastQCFastQC Report
Thu 2 Jun 2016
DRR015280_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015280_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7302884
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264060.3615831772762651No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209990.2875439346975798No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122770.16811166656898838No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA187550.051.815951
GTATCAA352700.041.672721
ATCAACG447000.032.5523833
TCAACGC450950.032.20894
TATCAAC465450.031.3116022
CAACGCA494000.029.365785
AACGCAG508750.028.559326
ACGCAGA539850.026.6446637
CGCAGAG538850.026.469628
GCAGAGT564650.024.8492379
GTACATG407600.024.5134961
TACATGG387550.024.2228412
GTGGTAT78850.023.8282741
ACATGGG402250.022.7090553
GAGTACT300550.022.23735212-13
TGGTATC84150.021.6284922
AGTACTT311400.020.69550512-13
CAGAGTA553650.020.52745810-11
GTACTTT323000.020.47258614-15
AGAGTAC522500.020.45383510-11
Produced by FastQC (version 0.11.5)