FastQCFastQC Report
Thu 2 Jun 2016
DRR015280_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015280_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7302884
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251250.34404216197326976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207200.28372352621238406No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT135020.18488586153086917No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121450.16630416147921834No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG86370.1182683443965425RNA PCR Primer, Index 23 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT84430.11561185964339567RNA PCR Primer, Index 23 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA191500.054.835011
CGTAGAG11850.049.179478
CAACGCG7400.047.1274954
ACGCGGA8000.043.5932356
GTATCAA362450.042.80471
CAACGAA19150.041.214995
AACGAAG19400.040.92046
AACGGAG14350.039.652026
CAACGCT9800.038.3954964
AACGCGG9400.037.5887955
AACGCCG10300.036.97745
TCAACGG15650.036.9444054
ACGTAGA17400.035.3387377
TCAACGT17700.034.221044
ATCAACG452950.034.040993
ACGGAGA16900.033.1259737
ACGAAGA24300.032.480127
TATCAAC489400.031.6443542
TCAACGC437950.030.9093574
AACGTAG20000.030.2858286
Produced by FastQC (version 0.11.5)