FastQCFastQC Report
Thu 2 Jun 2016
DRR015279_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015279_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8984863
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332310.36985538900259246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263140.2928703531706605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155680.17326919731552945No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA205800.057.324921
GTATCAA390550.043.074911
ATCAACG510950.032.560413
TCAACGC512650.032.3842934
TATCAAC519400.032.2210732
CAACGCA560050.029.635645
AACGCAG576800.028.8223886
GTGGTAT74700.027.8379081
ACGCAGA619850.026.5538887
CGCAGAG627400.026.0405968
TGGTATC78750.026.0069852
GTACATG457950.025.23371
TACATGG449000.024.5134942
GCAGAGT655700.024.4934279
ACATGGG474900.022.4473763
GAGTACT380950.021.38387312-13
CAGAGTA634800.021.01033610-11
CATGGGG310000.020.9106674
AGAGTAC615850.020.722310-11
GTACTTT396150.020.26405314-15
Produced by FastQC (version 0.11.5)