FastQCFastQC Report
Thu 2 Jun 2016
DRR015279_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015279_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8984863
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309520.3444905058652536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252840.28140662801425015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151160.16823851404300766No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT116290.12942879596494683No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA216750.055.9401321
CAACGCG7850.046.1813434
GTATCAA403150.043.3686561
CAACGCT8950.042.0436024
CGTAGAG15050.041.1643268
AACGAAG27550.037.144736
AACGGAG20600.034.5285576
ATCAACG513250.033.928463
CAACGAA29700.033.528745
TATCAAC537400.032.4619182
TCAACGG21950.031.7773844
ACGTAGA21850.031.7131587
AACGCCG12100.031.0987175
TCAACGC489600.030.8551374
ACGCGGA11950.030.7210486
CAACGCA501250.030.1383425
AACGCGG12050.030.0852765
ACGAAGA34300.029.8349087
GTGGTAT78650.029.8139741
CAACGTA23000.029.5289385
Produced by FastQC (version 0.11.5)