FastQCFastQC Report
Thu 2 Jun 2016
DRR015278_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015278_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7630325
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266680.3495001851166235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212620.27865130253298515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126470.16574654421666182No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA177350.052.9272961
GTATCAA334200.043.3587041
ATCAACG429600.033.343693
TCAACGC430250.033.2844854
TATCAAC443300.032.356512
CAACGCA458200.031.2543495
AACGCAG474400.030.2828186
ACGCAGA518150.027.4190657
CGCAGAG523600.026.8832938
GCAGAGT547850.025.3030329
GTACATG391600.024.2961331
TACATGG377300.024.0142692
GTGGTAT74200.023.8502731
TGGTATC76400.022.5722772
TACCTGG54700.022.1021982
ACATGGG401850.021.8952063
GAGTACT306250.021.16158112-13
CAGAGTA533100.020.76751110-11
AGAGTAC506150.020.6643410-11
AGTACTT310150.020.27206812-13
Produced by FastQC (version 0.11.5)