FastQCFastQC Report
Thu 2 Jun 2016
DRR015278_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015278_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7630325
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263250.34500496374662937No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217390.28490267452565915No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131460.17228623944589516No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA191700.053.6505361
CAACGCG6850.050.247744
CGTAGAG12250.045.3324858
GTATCAA351650.043.1523741
ACGCGGA8800.041.199346
AACGAAG21250.039.737886
CAACGCT7950.035.7907184
TCAACGG15500.034.937854
ATCAACG439850.034.298143
CAACGAA24350.033.924965
AACGCCG9850.033.5461045
AACGCGG11100.032.2490925
GCGGAGT11600.032.046998
AACGGAG16900.032.043596
TATCAAC474000.032.0260052
TCAACGC420000.031.5350044
CAACGCA427900.030.9313475
AACGCAG443750.029.8885846
CAACGTA20100.029.6825
ACGAAGA28750.029.2118517
Produced by FastQC (version 0.11.5)