FastQCFastQC Report
Thu 2 Jun 2016
DRR015277_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015277_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7645507
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306540.4009413633392789No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242220.3168135219809491No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148220.1938654951202059No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA171600.057.057011
GTATCAA331400.043.9172131
ATCAACG434450.033.23463
TCAACGC439150.032.8611874
TATCAAC448250.032.2729152
CAACGCA470400.030.6589745
AACGCAG485400.029.6835986
GTGGTAT64700.028.9823881
ACGCAGA529900.026.8529017
CGCAGAG532850.026.4679688
TGGTATC69550.025.3929462
GTACATG383550.025.3012581
TACATGG366950.024.798052
GCAGAGT559700.024.6373799
ACATGGG389050.022.4525993
TACCTGG55800.021.6725962
GAGTACT329700.021.33995212-13
TATAACG8050.020.9751512
CAGAGTA546550.020.622810-11
AGAGTAC526150.020.3847810-11
Produced by FastQC (version 0.11.5)