FastQCFastQC Report
Thu 2 Jun 2016
DRR015277_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015277_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7645507
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300040.39243963807763177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241840.31631649804257583No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150940.19742314015277207No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA190000.057.6612551
CAACGCG7800.048.2303124
CGTAGAG12550.046.793678
GTATCAA364900.044.0746541
TCAACGG16250.040.37234
ACGCGGA9550.040.3530856
AACGCCG11600.039.5496675
AACGGAG17350.037.0193986
CAACGCT10300.036.9693374
CAACGAA21550.036.1911545
AACGAAG21900.035.8222476
AACGCGG10850.035.0953145
ATCAACG458750.034.7172623
GCGGAGT11250.033.033418
TATCAAC486550.032.8282172
TCAACGC440250.031.804314
CAACGCA446250.031.4280935
ACGTAGA20350.031.1111097
AACGCAG459800.030.6815286
GTGGTAT68600.030.2015571
Produced by FastQC (version 0.11.5)