FastQCFastQC Report
Thu 2 Jun 2016
DRR015276_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015276_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6490836
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233310.3594452240050434No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188360.2901937439183489No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114370.17620226423838162No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158200.051.111781
GTATCAA290900.040.275331
ATCAACG369650.031.3473683
TCAACGC373300.031.0081164
TATCAAC386950.029.9554562
CAACGCA403200.028.720395
AACGCAG414450.027.9847246
ACGCAGA446850.025.5527557
CGCAGAG448800.025.2524538
GTACATG326300.024.9901681
TACATGG309900.024.6558172
GTGGTAT71800.024.0731811
GCAGAGT472300.023.5239589
TGGTATC72850.022.918052
ACATGGG328050.022.5448173
GAGTACT268550.020.84678812-13
AGTACTT268800.020.41271212-13
AGAGTAC433150.020.38252610-11
CATGGGT63600.020.182694
TACCTGG47800.019.9454822
Produced by FastQC (version 0.11.5)