FastQCFastQC Report
Thu 2 Jun 2016
DRR015276_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015276_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6490836
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208780.3216534819243623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179900.2771599837062591No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104700.16130433737657215No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT73720.11357550860936864No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169000.051.8163071
CAACGCG6200.050.3411834
CGTAGAG9100.043.884498
ACGCGGA7250.041.785186
GTATCAA312450.041.5227171
AACGCCG6900.039.2481545
AACGCGG8200.038.6234975
AACGGAG12250.038.2190936
CAACGCT7750.037.903954
AACGAAG17200.037.360786
ATCAACG384400.033.4631273
GCGGAGT9200.033.4287538
CAACGAA20200.031.8121475
TATCAAC413300.031.2411382
TCAACGC371550.030.7107244
CAACGCA378750.030.1276195
TCAACGG15800.029.34074
AACGCAG391900.029.1635516
ACGAAGA22450.029.032767
ACGGAGA16800.028.1413087
Produced by FastQC (version 0.11.5)