FastQCFastQC Report
Thu 2 Jun 2016
DRR015275_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015275_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6137403
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219400.3574801915402981No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176780.2880371388354325No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT120180.19581572205703293No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107200.1746667116368275No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT68780.11206694427594213No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA407050.029.388341
GGTATCA281400.027.6165891
ATCAACG494650.024.0164493
TCAACGC497850.023.9192754
GTACATG312500.023.6568851
TATCAAC506800.023.512352
TACATGG298000.023.4378852
CAACGCA523250.022.7322565
AACGCAG535550.022.2356626
ACATGGG315200.021.4873583
GAGTACT252550.020.71727212-13
ACGCAGA569200.020.7001977
AGAGTAC415750.020.68838110-11
AGTACTT256900.020.64983412-13
CGCAGAG572300.020.4549378
TACCTGG43950.020.0335482
TATAACG5700.020.0089652
GTACTTT263000.019.5991314-15
GCAGAGT586350.019.4992379
CATGGGG174600.018.8496324
Produced by FastQC (version 0.11.5)