FastQCFastQC Report
Thu 2 Jun 2016
DRR015275_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015275_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6137403
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215220.35066949326938446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178050.29010641797515985No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT116700.19014557134344934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106320.1732328804218983No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT65120.1061035099047594No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG5450.047.1383864
CGTAGAG9600.045.4001168
AACGAAG17150.041.9973876
AACGCCG8950.036.905935
CAACGCT6900.036.5676274
CAACGAA20000.035.783385
ACGCGGA7500.035.477546
AACGGAG14500.035.1189656
TCAACGG15000.033.3363764
AACGCGG7850.033.311335
ACGAAGA24200.030.1417527
GCGGAGT9300.030.092038
ACGGAGA17000.028.8749717
GGTATCA318650.028.4947051
ACGTAGA16500.028.0817497
GTATCAA453000.027.8441831
CGAAGAG26050.027.2978558
CAACGTA16300.027.0190685
AACGTAG17300.025.9876296
TCAACGA27750.025.9549274
Produced by FastQC (version 0.11.5)