FastQCFastQC Report
Thu 2 Jun 2016
DRR015274_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015274_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6259549
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255450.40809649385283187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208570.33320291925185025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122060.19499807414240228No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159050.057.161631
GTATCAA312200.044.112451
ATCAACG405200.033.6561433
TCAACGC408500.033.4760784
TATCAAC415700.032.9270062
CAACGCA444050.030.888055
AACGCAG455500.030.1515856
ACGCAGA490600.027.706247
GTGGTAT61000.027.5200311
CGCAGAG496800.027.1335038
TGGTATC62650.025.7077852
GCAGAGT517450.025.6198719
GTACATG383700.023.8489951
TACATGG375700.023.3290582
GAGTACT273700.021.78301612-13
CAGAGTA499450.021.12343410-11
AGTACTT270700.021.05807312-13
ACATGGG402850.020.9939583
GTACTTT281250.020.87416614-15
TATAACG6850.020.6481972
Produced by FastQC (version 0.11.5)