FastQCFastQC Report
Thu 2 Jun 2016
DRR015274_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015274_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6259549
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227510.36346069021905575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192640.30775380143202014No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT131320.20979147219711836No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114900.1835595503765527No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT76250.12181388786955737RNA PCR Primer, Index 31 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA173450.058.1375921
CAACGCG6150.045.5154574
GTATCAA324050.042.118921
CGTAGAG10850.040.179558
AACGGAG13700.039.8597076
ACGTAGA11850.037.563077
ACGCGGA7400.037.2072226
AACGCGG7650.037.191015
AACGTAG12150.037.0132756
CAACGTA12050.036.5587965
AACGAAG22950.033.9917836
ACGCCGA10450.032.934626
ATCAACG411400.032.8689543
GCGGAGT8950.031.789228
TATCAAC432250.031.4262142
AACGCCG11550.030.9899145
ACGAAGA26050.030.1228547
TCAACGC393400.030.106334
CAACGCA403150.029.3443155
TCAACGG18550.029.1905544
Produced by FastQC (version 0.11.5)