FastQCFastQC Report
Thu 2 Jun 2016
DRR015273_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015273_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7737697
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208620.2696151064069839No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164260.21228538672424108No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99090.1280613598594No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156750.050.3630871
GTATCAA289500.041.588971
ATCAACG370150.032.0440563
TCAACGC373400.031.8293934
TATCAAC383850.031.077062
CAACGCA411700.028.8021875
AACGCAG427200.027.8741916
ACGCAGA449700.026.108897
CGCAGAG449700.025.920218
GTACATG353650.025.456361
TACATGG342950.025.0333292
GCAGAGT474550.024.141049
ACATGGG360750.023.1389583
GTGGTAT74600.022.1305681
TGGTATC73800.020.95432
AGAGTAC433800.020.76624110-11
GAGTACT265800.020.3863312-13
CATGGGG219900.020.368814
CAGAGTA460600.019.83947410-11
GTACCGT3700.019.6840326
Produced by FastQC (version 0.11.5)