FastQCFastQC Report
Thu 2 Jun 2016
DRR015273_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015273_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7737697
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204250.26396743113616367No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164890.21309958247266594No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT103270.13346348403148897No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98770.12764780011416832No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA178900.051.967441
CAACGCG6000.045.882454
CGTAGAG10400.041.4717568
GTATCAA324000.041.1866761
AACGAAG18600.036.0157286
ACGCGGA7700.035.7530256
CAACGAA19350.034.619775
ATCAACG395100.033.5143853
TCAACGG14600.032.0548674
TATCAAC425300.031.2364182
TCAACGC380100.030.7090114
AACGCCG9850.029.8123385
CAACGCA392600.029.7784065
CAACGCT9650.029.4788844
AACGCAG407150.028.8381986
GCGGAGT10000.028.447868
AACGGAG16450.028.1713566
AACGCGG11150.025.5134475
ACGCAGA456400.025.4447867
CAACGTA17550.025.3598635
Produced by FastQC (version 0.11.5)