FastQCFastQC Report
Thu 2 Jun 2016
DRR015272_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015272_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6451353
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252420.39126676218151446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205050.31784030419665454No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123620.19161871936010944No Hit
CATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTCGCCGT121050.18763505887834692Illumina Single End PCR Primer 1 (95% over 24bp)
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC68720.10652029116992978No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153500.057.2480471
GTATCAA301250.043.8690951
ATCAACG386950.033.875963
TCAACGC389850.033.6387754
TATCAAC398150.033.0525252
CAACGCA421900.031.0730695
AACGCAG436950.030.0236436
ACGCAGA469750.027.5318227
CGCAGAG473350.027.1526038
GTGGTAT57800.026.7460211
TGGTATC58350.026.4229552
GTACATG340350.025.4976621
GCAGAGT495000.025.4135519
TACATGG324750.024.9742
ACATGGG348050.022.8457433
GAGTACT294750.021.9647612-13
TACCTGG44550.021.6043032
CATGGGT62200.021.0032234
CAGAGTA485900.020.9737710-11
GTACTTT306500.020.81019414-15
Produced by FastQC (version 0.11.5)