FastQCFastQC Report
Thu 2 Jun 2016
DRR015272_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015272_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6451353
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221630.3435403395225777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190700.2955969081214437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113420.17580808242860063No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT74430.11537114772668616No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA166000.056.543411
CGCGTAT1700.051.2817739
CGTAGAG10150.050.1782238
CAACGCG6550.049.735354
ACGCGGA7200.043.9708376
GTATCAA311650.042.655891
TCAACGG12550.041.678234
AACGCGG8150.040.5343325
AACGGAG12850.040.3481376
AACGCCG9750.038.1178635
ATCGCGT1950.037.647277
AACGAAG18700.036.8042346
CAACGCT7650.035.9863624
CAACGAA19250.035.2759865
ATCAACG395400.033.3950843
ACGTAGA16400.032.4535567
TATCAAC421000.031.447062
TCAACGC382500.030.288524
ACGAAGA22950.030.1885627
GTGGTAT62150.029.7506831
Produced by FastQC (version 0.11.5)