FastQCFastQC Report
Thu 2 Jun 2016
DRR015271_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015271_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7081446
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234560.3312317851467059No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193170.27278327053542456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112730.1591906511749154No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA164350.053.758031
GTATCAA314100.043.9279441
ATCAACG412150.033.1309133
TCAACGC414400.032.9984634
TATCAAC418550.032.7743572
CAACGCA456900.029.9489335
AACGCAG470650.029.1027876
ACGCAGA498350.027.147417
CGCAGAG501500.026.7625948
GTGGTAT64450.026.5394421
GCAGAGT526250.025.0370759
GTACATG369400.024.58431
TACATGG357200.024.3166392
TGGTATC70000.023.8523482
ACATGGG373000.022.636153
GAGTACT294450.021.7709112-13
AGTACTT301400.021.05001412-13
AGAGTAC493650.020.84733810-11
GTACTTT309500.020.49081814-15
CAGAGTA512600.020.41391810-11
Produced by FastQC (version 0.11.5)