FastQCFastQC Report
Thu 2 Jun 2016
DRR015271_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015271_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7081446
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234410.33101996400170247No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199440.28163739439656815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115270.1627774892303069No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT90340.1275728149307359No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG5700.058.755614
GGTATCA179200.057.2018971
CGTAGAG12100.045.5001378
GTATCAA337500.043.6219251
ACGCGGA7800.042.9367946
AACGCGG8650.040.3087045
AACGGAG13300.036.9091036
AACGAAG22900.036.061056
AACGCCG9650.035.1808055
TCAACGG13550.034.8738024
CAACGAA23100.034.755815
ATCAACG425600.034.2841763
CAACGCT9300.034.0382734
TATCAAC444500.032.905672
AACGTAG17400.032.4307676
CAACGTA17750.032.049755
TCAACGC408000.031.4509434
ACGAAGA26250.030.9346587
CAACGCA422000.030.4401595
GTGGTAT65850.030.1511341
Produced by FastQC (version 0.11.5)