FastQCFastQC Report
Thu 2 Jun 2016
DRR015270_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015270_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7540078
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276650.36690601874410317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236260.31333893362906856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132000.17506450198525797No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA183300.055.760261
GTATCAA341600.041.9021151
ATCAACG436700.032.521983
TCAACGC437350.032.466044
TATCAAC455250.031.2679962
CAACGCA467950.030.4016085
AACGCAG482600.029.4975916
GTGGTAT70100.029.356061
ACGCAGA523750.027.0118067
TGGTATC74500.026.6481842
CGCAGAG528100.026.5254468
GCAGAGT553850.024.8566769
GTACATG391600.023.4785671
TACATGG376000.023.0864582
GAGTACT318800.021.65606512-13
ACATGGG394850.021.3623433
TACCTGG58900.021.2309742
CAGAGTA545150.021.04314610-11
GTACTTT329900.020.60961214-15
AGAGTAC524750.020.18791810-11
Produced by FastQC (version 0.11.5)