FastQCFastQC Report
Thu 2 Jun 2016
DRR015270_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015270_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7540078
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278320.3691208499434621No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242840.3220656338037883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134080.17782309413775296No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA193350.056.976661
CAACGCG6700.052.7300954
GTATCAA361300.043.1285441
TCAACGG17500.040.6383674
ACGCGGA8500.039.9443556
AACGAAG21850.039.0574726
AACGCGG9450.038.841935
AACGGAG19150.037.6160746
CAACGAA23350.036.5484245
ATCAACG449400.034.6329573
CAACGCT8250.033.9248924
AACGCCG11500.032.715885
TATCAAC482800.032.2543872
GTGGTAT71850.031.4887541
ACGGAGA23100.031.3825157
TCAACGC427250.031.3361644
GCGGAGT11400.030.9913228
CAACGCA438500.030.4485075
AACGCAG451350.029.5816356
TGGTATC75300.029.5263232
Produced by FastQC (version 0.11.5)