FastQCFastQC Report
Thu 2 Jun 2016
DRR015269_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015269_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9162604
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342160.3734309591465483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293980.32084765422580747No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164620.17966508210984564No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA274950.046.7653771
GTATCAA469300.037.4067571
ATCAACG590500.029.534393
TCAACGC593150.029.4911944
TATCAAC596450.029.286472
CAACGCA623400.028.1039665
AACGCAG640750.027.3854486
ACGCAGA708350.024.4390567
CGCAGAG715800.023.9881848
GTACATG492000.023.6202581
TACATGG474450.023.3740042
GCAGAGT744300.022.5453079
TATAACG7150.022.3489992
TACCTGG67800.022.0198382
ACATGGG495450.021.7338833
GAGTACT386250.021.71640612-13
GTGGTAT136650.020.5426671
GTACTTT404150.020.52912914-15
AGTACTT388900.020.34580812-13
AGAGTAC652650.019.6215910-11
Produced by FastQC (version 0.11.5)