FastQCFastQC Report
Thu 2 Jun 2016
DRR015269_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015269_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9162604
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT403510.4403879071931953No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT396500.43273724369185873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350990.3830679575369622No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT224910.24546515379252448RNA PCR Primer, Index 33 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195670.21355282843174275No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG174350.19028433401683625RNA PCR Primer, Index 33 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT124640.13603119811791495RNA PCR Primer, Index 33 (95% over 24bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93770.10233990249933315No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA298400.046.8108441
GTATCAA508750.037.304271
ATCAACG637000.029.6594523
TCAACGC640400.029.4808374
TATCAAC650150.029.1218992
CAACGCA663750.028.4935765
AACGCAG682500.027.7507846
ACGCAGA765650.024.5290587
CGCAGAG774900.024.035988
TACCTGG69850.023.7121242
GTACATG490050.023.0338651
GCAGAGT801950.022.7600529
TACATGG473550.022.6767942
GAGTACT439550.021.82454112-13
CATGGGT88800.021.4656544
GTGGTAT149350.021.2451881
ACATGGG492100.021.2440513
TATAACG8000.021.0449182
GTACTTT454450.020.8940814-15
TGGTATC149600.020.7437972
Produced by FastQC (version 0.11.5)