FastQCFastQC Report
Thu 2 Jun 2016
DRR015268_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015268_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7234514
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296810.41026943896991563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254140.35128828280655755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140020.19354444541817184No Hit
GTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGT75550.1044299589440286No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG75070.10376647277204801No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA271400.044.7794271
GTATCAA455850.037.2524571
ATCAACG571350.029.6105163
TCAACGC578650.029.2347584
TATCAAC598200.028.2938422
CAACGCA606800.027.9460535
AACGCAG623950.027.2508816
TACCTGG47350.025.8352932
ACGCAGA682100.024.6325657
CGCAGAG684500.024.4204188
GTACATG508350.023.6915651
GCCGTAT23300.023.62826590-91
TATAACG6650.023.3375822
TACATGG477300.023.1909682
GCAGAGT709450.023.0817349
CATGGGT88400.022.5559754
GAGTACT338150.022.23486112-13
ACATGGG495950.021.5943243
GTACTTT350100.021.23512514-15
AGTACTT335400.020.86390712-13
Produced by FastQC (version 0.11.5)