FastQCFastQC Report
Thu 2 Jun 2016
DRR015268_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015268_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7234514
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT471400.6515987113992731No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330010.4561605658652399No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290380.40138148879109226No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT242920.3357792935365112RNA PCR Primer, Index 31 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158030.21843899949602696No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG145890.20165832839635117RNA PCR Primer, Index 31 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT134100.18536144929707787RNA PCR Primer, Index 31 (95% over 24bp)
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG79000.1091987658051391No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75110.10382176328637971No Hit
GTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGT74540.10303387345715276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA285050.044.720651
GTATCAA468000.036.3147621
ATCAACG595150.028.4501343
TCAACGC603450.027.9834384
TATCAAC625050.027.1940882
CAACGCA625850.027.0637025
AACGCAG640550.026.4781276
TATAACG8150.026.2375132
TACCTGG49500.024.9084422
ACGCAGA709650.023.6947547
CATGGGT89300.023.5865254
CGCAGAG714300.023.438118
GTACATG508800.022.9134311
TACATGG476300.022.581322
GCAGAGT734750.022.420459
TAACGCA9850.022.1686574
GAGTACT361400.022.09915212-13
AGTACTT359500.021.2921312-13
ACATGGG495050.021.0803953
GTACTTT373300.021.04901514-15
Produced by FastQC (version 0.11.5)