FastQCFastQC Report
Thu 2 Jun 2016
DRR015267_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015267_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10050937
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284950.28350590596677705No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243400.24216647661805063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135890.13520132501079252No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA243100.051.743621
GTATCAA436400.039.9353561
ATCAACG554200.031.4103283
TCAACGC560100.031.092544
TATCAAC566100.030.7045062
CAACGCA601550.028.9954955
AACGCAG621650.028.1165526
ACGCAGA670400.025.679617
CGCAGAG675650.025.2650998
GTGGTAT105400.024.1720641
GTACATG492900.023.954651
GCAGAGT698050.023.8169299
TACATGG472200.023.540792
TGGTATC111400.022.42482
ACATGGG497150.021.6588233
GAGTACT372850.021.19675612-13
CAGAGTA679050.020.31232310-11
GTACTTT381800.020.29418614-15
TACCTGG65750.019.9348342
AGAGTAC640950.019.87994610-11
Produced by FastQC (version 0.11.5)