FastQCFastQC Report
Thu 2 Jun 2016
DRR015267_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015267_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10050937
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT575670.5727525702330041No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335690.3339887614458234No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT315570.3139707273063198RNA PCR Primer, Index 23 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291650.29017195113251626No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG286960.28550571951649883RNA PCR Primer, Index 23 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT171020.17015329018578068RNA PCR Primer, Index 23 (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163390.16256195815375224No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA250300.050.9042741
GTATCAA457900.040.0808031
ATCAACG578600.031.5996113
TCAACGC582500.031.4427174
TATCAAC591900.030.9115542
CAACGCA613250.029.9131285
AACGCAG634200.028.960866
ACGCAGA699200.025.9952667
CGCAGAG702500.025.6584118
GTGGTAT117850.024.678821
TGGTATC115250.024.5180422
GCAGAGT731750.024.1353979
GTACATG495000.023.124631
TACATGG477000.022.7130832
GAGTACT403950.021.49460212-13
ACATGGG497900.020.9875163
TACCTGG67000.020.577992
GTACTTT416500.020.57497214-15
AGTACTT404200.020.38951512-13
CAGAGTA713550.020.17982510-11
Produced by FastQC (version 0.11.5)