FastQCFastQC Report
Thu 2 Jun 2016
DRR015266_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015266_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10444762
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT375250.35927099152666186No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311090.2978430719627695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183800.17597337306489128No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA244100.055.1595761
GTATCAA466200.043.0869521
ATCAACG602800.033.260773
TATCAAC609200.032.898632
TCAACGC610250.032.8142134
CAACGCA647350.030.8984535
AACGCAG669850.030.0032966
GTGGTAT99650.029.4968241
TGGTATC103750.027.2380312
ACGCAGA734600.026.9904847
CGCAGAG740850.026.5423438
GCAGAGT770200.024.7811559
GTACATG539550.023.7537441
TACATGG523600.023.4108832
ACATGGG551200.021.6017113
GAGTACT427550.021.36297612-13
CAGAGTA746900.020.74490710-11
AGTACTT430250.020.47810412-13
AGAGTAC720700.020.4005910-11
TACCTGG79700.020.3349552
Produced by FastQC (version 0.11.5)